Sars-Cov Nonstructural Protein 13 Antibody
IF Microscopy, Immunoprecipitation, Western Blot
Rabbit
SARS Coronavirus nsp13 protein
Antiserum
Polyclonal
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-term
rabbit anti-Sars-Cov Nonstructural Protein 13 Antibody, Replicase polyprotein 1a, pp1a antibody, Growth factor-like peptide
The coronavirus nonstructural protein 13 (nsp13) is one of the SARS-Coronavirus replicase cleaving products encoded by ORF1b. Nsp13 is thought to be part of the viral replication complex, which is associated with intracellular membranes. Nsp13 contains a C-terminal NTPase/Helicase domain and an N-terminal putative zinc-binding motif. Anti-SARS-CoV Nonstructural Protein 13 (nsp13) Antibody is useful for researchers interested in viral research. This antibody is suitable for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 67 kDa in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C. Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mMHEPES/2% FCS. Cells were fixed with PBS/3% PFA. After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended sto
