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  3. A700-041-T
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Rabbit anti-CTCF Recombinant Monoclonal Antibody [BLR041F]

Catalogue number:
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A700-041-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00
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Estimated delivery Fri 26 Apr
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Applications:

WB, IP, IHC, ICC, Flow Cyt, SW-Size

Antibody Host:

Rabbit

Target:

CTCF

Species Reactivity:

Human; Mouse

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 650 and 700

Alternative Names:

CTCFL paralog; MRD21; CCCTC-binding factor (zinc finger protein); CCCTC-binding factor; 11-zinc finger protein; 11 zinc finger transcriptional repressor; transcriptional repressor CTCF

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Product Description:

Rabbit anti-CTCF Recombinant Monoclonal Antibody [BLR041F] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human CTCF by western blot.
Detection of human CTCF by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human CTCF by western blot
Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used for IP at 20 µl/mg lysate. CTCF was also immunoprecipitated by rabbit anti-CTCF antibody A300-543A. For blotting immunoprecipitated CTCF, A700-041-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
Detection of human CTCF by immunohistoch
Detection of human CTCF by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human CTCF by immunocytoche
Detection of human CTCF by immunocytochemistry. Sample: FFPE section of MCF-7 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of mouse CTCF by immunohistoch
Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
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