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Rabbit anti-CTCF Recombinant Monoclonal Antibody [BLR041F]

Catalogue number:
A700-041-T
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
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Recombinant antibody was purified from cell culture supernatant.

Product Type:

Antibodies

Alternative Names:

11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), CTCFL paralog, MRD21, transcriptional repressor CTCF

Species Reactivity:

Human, Mouse

Immunogen:

between 650 and 700

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Formulation:

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide

Antibody Type:

Recombinant Monoclonal

UNSPSC Code:

12352203

Detection of human CTCF by western blot.

Detection of human CTCF by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.

Detection of human CTCF by western blot

Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used for IP at 20 µl/mg lysate. CTCF was also immunoprecipitated by rabbit anti-CTCF antibody A300-543A. For blotting immunoprecipitated CTCF, A700-041-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

Detection of human CTCF by immunohistoch

Detection of human CTCF by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Detection of human CTCF by immunocytoche

Detection of human CTCF by immunocytochemistry. Sample: FFPE section of MCF-7 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Detection of mouse CTCF by immunohistoch

Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.