Rabbit anti-CTCF Recombinant Monoclonal Antibody [BLR041F]
Recombinant antibody was purified from cell culture supernatant.
- Data sheet/protocol: View or download
- MSDS: View or download
- Applications: WB, IP, IHC, ICC
Antibodies
11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), CTCFL paralog, MRD21, transcriptional repressor CTCF
Human, Mouse
between 650 and 700
2 - 8°C
Rabbit
Whole IgG
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Recombinant Monoclonal
12352203
Detection of human CTCF by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041-T lot 1) used for IP at 20 µl/mg lysate. CTCF was also immunoprecipitated by rabbit anti-CTCF antibody A300-543A. For blotting immunoprecipitated CTCF, A700-041-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
