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Rabbit anti-IDO1 Recombinant Monoclonal Antibody [BLR040F]

Catalogue number:
A700-040-T
Size:
20 µl (2 blots)
Product is available in:
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Recombinant antibody was purified from cell culture supernatant.

Product Type:

Antibodies

Alternative Names:

IDO, IDO-1, INDO, indolamine 2,3 dioxygenase, indole 2,3-dioxygenase, indoleamine 2,3-dioxygenase 1, indoleamine-pyrrole 2,3-dioxygenase

Species Reactivity:

Human

Immunogen:

residues 1-403 (full length)

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Formulation:

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide

Antibody Type:

Recombinant Monoclonal

UNSPSC Code:

12352203

Detection of human IDO1 by western blot.

Detection of human IDO1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with IFN-gamma (+) or mock treated (-). Antibody: Rabbit anti-IDO1 recombinant monoclonal antibody [BLR040F] (A700-040-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Vinculin (A302-535A).

Detection of human IDO1 by western blot

Detection of human IDO1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with IFN-gamma. Antibodies: Rabbit anti-IDO1 recombinant monoclonal antibody [BLR040F] (A700-040-T lot 1) used for IP at 15 µl per reaction. IDO1 was also immunoprecipitated by rabbit anti-IDO1 antibody (A700-031). For blotting immunoprecipitated IDO1, A700-040-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.