Antibody was affinity purified using an epitope specific to Rad17 immobilized on solid support. The epitope recognized by A305-788A-T maps to a region between residue 564 and 614 of human RAD17 checkpoint clamp loader component using the numbering given in entry O75943 (GeneID 5884). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, HRAD17, R24L, RAD1 homolog, RAD17 homolog, Rad17-like protein, RAD17SP, RAD24, RF-C activator 1 homolog, RF-C/activator 1 homolog
between 564 and 614
2 - 8°C
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Detection of human Rad17 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad17 antibody A305-788A-T (lot A305-788A-T-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Rad17 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rad17 antibody A305-788A-T (lot A305-788A-T-1) used for IP at 40 µl per reaction. Rad17 was also immunoprecipitated by rabbit anti-Rad17 antibody BL239. For blotting immunoprecipitated Rad17, A305-788A-T was used at 1:100. Detection: Chemiluminescence with an exposure time of 30 seconds.