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Rabbit anti-CNOT1 Antibody, Affinity Purified

Catalogue number:
A305-787A-T
Size:
10 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£122.00 Estimated delivery Fri 26 Apr
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

CNOT1

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 1400 and 1450

Alternative Names:

CDC39; NOT1H; NOT1 (negative regulator of transcription 1, yeast) homolog; NOT1; hNOT1; CCR4-NOT transcription complex subunit 1; CCR4-associated factor 1; adrenal gland protein AD-005; AD-005; negative regulator of transcription subunit 1 homolog

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Product Description:

Rabbit anti-CNOT1 Antibody, Affinity Purified - 10 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse CNOT1 by we
Detection of human and mouse CNOT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CNOT1 antibody A305-787A-T (lot A305-787A-T-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human CNOT1 by western blot
Detection of human CNOT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CNOT1 antibody A305-787A-T (lot A305-787A-T-1) used for IP at 50 µl per reaction. CNOT1 was also immunoprecipitated by rabbit anti-CNOT1 antibody BL23426. For blotting immunoprecipitated CNOT1, A305-787A-T was used at 1:40. Detection: Chemiluminescence with an exposure time of 30 seconds.
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