Antibody was affinity purified using an epitope specific to CNOT1 immobilized on solid support. The epitope recognized by A305-787A-M maps to a region between residue 1400 and 1450 of human CCR4-NOT transcription complex subunit 1 using the numbering given in entry A5YKK6.2 (GeneID 23019). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
AD-005, adrenal gland protein AD-005, CCR4-associated factor 1, CCR4-NOT transcription complex subunit 1, CDC39, hNOT1, negative regulator of transcription subunit 1 homolog, NOT1, NOT1 (negative regulator of transcription 1, yeast) homolog, NOT1H
between 1400 and 1450
2 - 8°C
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Detection of human and mouse CNOT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CNOT1 antibody A305-787A-M (lot A305-787A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human CNOT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CNOT1 antibody A305-787A-M (lot A305-787A-M-1) used for IP at 50 µl per reaction. CNOT1 was also immunoprecipitated by rabbit anti-CNOT1 antibody BL23426. For blotting immunoprecipitated CNOT1, A305-787A-M was used at 1:40. Detection: Chemiluminescence with an exposure time of 30 seconds.