Antibody was affinity purified using an epitope specific to AMPK alpha 1 immobilized on solid support. The epitope recognized by A300-507A-M maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1 (GeneID 5562). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase, catalytic alpha-1 chain, ACACA kinase, acetyl-CoA carboxylase kinase, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK,
between 350 and 400
2 - 8°C
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody A300-507A-M (lot A300-507A-M-3) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.