Antibody was affinity purified using an epitope specific to SMG1 immobilized on solid support. The epitope recognized by A300-394A-M maps to a region between residues 2925 and 2975 of human PI-3-Kinase-related kinase SMG1 using the numbering given in entry NP_055907.3 (GeneID 23049). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
61E3.4, ATX, hSMG-1, lambda/iota protein kinase C-interacting protein, lambda-interacting protein, LIP, PI-3-kinase-related kinase SMG-1, serine/threonine-protein kinase SMG1, SMG-1, smg-1 homolog, phosphatidylinositol 3-kinase-related kinase, SMG1 phosph
between 2925 and 2975
2 - 8°C
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Detection of human SMG1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody A300-394A-M (lot A300-394A-M-3) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.