Antibody was affinity purified using an epitope specific to CSN4 immobilized on solid support. The epitope recognized by A300-013A-M maps to a region between residues 350 and the C-terminus (residue 406) of human COP9 constitutive photomorphogenic homolog subunit 4 using the numbering given in entry NP_057213.2 (GeneID 51138). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
COP9 constitutive photomorphogenic homolog subunit 4, COP9 constitutive photomorphogenic-like protein subunit 4, COP9 signalosome complex subunit 4, CSN4, JAB1-containing signalosome subunit 4, SGN4, signalosome subunit 4, testis tissue sperm-binding prot
between 350 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse CSN4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSN4 antibody A300-013A-M (lot A300-013A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.