CD antigen CD366, CD366, HAVcr-2, hepatitis A virus cellular receptor 2, kidney injury molecule-3, KIM-3, T cell immunoglobulin mucin 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immun
residues 22-201 (ECD)
2 - 8°C
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Detection of human TIM3 by western blot. Samples: Whole cell lysate (50 µg) from SR, MJ, HDLM-2, MOLT-4, SUPM2, Hep-G2, KG-1, HeLa, RPMI-8226, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody [BLR033F] (A700-033-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).