Doxycycline is A synthetic derivative of oxytetracycline. Acts as a broad spectrum antibiotic and matrix metallo-proteinases (MMP) inhibitor. Useful in studies involving wound healing and tissue remodeling. Common side effects include diarrhea, nausea, vomiting, a red rash, and an increased risk of a sunburn. Use during pregnancy or in young children may result in permanent problems with the teeth including changes in their color. Standard techniques/instruments (HPLC or GC-MS) are utilised to detect Doxycycline. However, these techniques are complex, expensive, laborious, and time-consuming. Immunoassay techniques, such as ELISAs are commonly preferred as a simple, reliable and rapid method for the quantification of Doxycycline in various samples. BioVision’s Doxycycline ELISA Kit is a competitive-based ELISA that can be used for the determination of this antibiotic in tissue, honey, milk and serum.