Tumor Necrosis Factor-α (TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. TNF is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer. BioVision’s TNF-alpha ELISA kit is a sandwich ELISA assay for the quantitative measurement of TNF-alpha in Human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of TNF-alpha captured from the samples.