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EZClick™ Global Protein Synthesis Assay Kit

Supplier:
Catalogue number:
K467-100
Size:
100 assays
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£569.00 Estimated delivery Wed 28 Apr
Shipping is calculated in checkout

Protein synthesis is a tightly regulated process and many critical controls in gene expression occur at the level of translation to ensure that production of specific cellular proteins is quickly turned on/off under specific conditions (heat shock, starvation, etc). It is essential in cell growth, proliferation, signaling, differentiation or death, therefore, the identity and amount of the synthesised proteins are critical in determining the physiological state of the cell. Methods of detection and characterisation of nascent proteins, or changes in spatial and temporal protein expression/degradation patterns during disease, drug treatments or environmental changes are important for assessment of cytotoxicity. Biovision’s EZClick™ Global Protein Synthesis Assay Kit utilises a novel chemical method based on alkyne analog of puromycin, O-Propargyl-puromycin (OP-puro). OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide (Ex/Em= 494/521 nm). OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. We provide sufficient materials for 100 simple and specific assays to detect nascent proteins synthesised under various physiological conditions in the real-time, and Cycloheximide, an inhibitor of protein synthesis that serves as a control

  • Data sheet/protocol: View or download
  • MSDS: View or download
  • Applications: This assay provides a convenient and accurate procedure to measure de novo protein synthesized in biological samples.
Product Type:

Biochemical and cellular assays

Storage Temp:

-20°C

UNSPSC Code:

41116133

EZClick™ Global Protein Synthesis Assay

EZClick™ Global Protein Synthesis Assay Kit