Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. CF® dyes are Biotium's line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Orange fluorescent CF®543 has excitation/emission at 541/560 nm. Kit Components:
- CF®543 tyramide stock solution
- HRP Goat anti-rabbit IgG
- BSA for blocking
- Amplification buffer
- Hydrogen peroxide 30% stablized solution
- Data sheet: View or download
Storage Temperature:
F