Rabbit anti-HIGD1A Antibody, Affinity Purified
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Rabbit anti-HIGD1A Antibody, Affinity Purified - 100 µg
Antibodies
HIG1, HIG1 domain family member 1A, mitochondrial, HIG1 domain family, member 1A, hypoxia inducible gene 1, hypoxia-inducible gene 1 protein, RCF1 homolog A, RCF1a
Human
Between 1 and 50
2 - 8°C
Rabbit
Whole IgG
Polyclonal
12352203
Detection of human HIGD1A by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HIGD1A antibody A305-549A (lot A305-549A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human HIGD1A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HIGD1A antibody A305-549A (lot A305-549A-1) used for IP at 6 µg per reaction. HIGD1A was also immunoprecipitated by rabbit anti-HIGD1A antibody BL22025. For blotting immunoprecipitated HIGD1A, A305-549A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.