Rabbit anti-NGLY1 Antibody, Affinity Purified
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Rabbit anti-NGLY1 Antibody, Affinity Purified - 100 µg
Antibodies
CDDG, CDG1V, hPNGase, N-glycanase 1, peptide:N-glycanase, peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase, PNG1, PNGase
Human
Between 250 and 300
2 - 8°C
Rabbit
Whole IgG
Polyclonal
12352203

Detection of human NGLY1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NGLY1 antibody A305-547A (lot A305-547A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human NGLY1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NGLY1 antibody A305-547A (lot A305-547A-1) used for IP at 6 µg per reaction. NGLY1 was also immunoprecipitated by rabbit anti-NGLY1 antibody BL21982. For blotting immunoprecipitated NGLY1, A305-547A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.