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Rabbit anti-NGLY1 Antibody, Affinity Purified

Catalogue number:
A305-547A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£383.00 Estimated delivery Wed 13 Jul
Shipping is calculated in checkout

Rabbit anti-NGLY1 Antibody, Affinity Purified - 100 µg

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

CDDG, CDG1V, hPNGase, N-glycanase 1, peptide:N-glycanase, peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase, PNG1, PNGase

Species Reactivity:

Human

Immunogen:

Between 250 and 300

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human NGLY1 by western blot

Detection of human NGLY1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NGLY1 antibody A305-547A (lot A305-547A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human NGLY1 by western blot

Detection of human NGLY1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NGLY1 antibody A305-547A (lot A305-547A-1) used for IP at 6 µg per reaction. NGLY1 was also immunoprecipitated by rabbit anti-NGLY1 antibody BL21982. For blotting immunoprecipitated NGLY1, A305-547A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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