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Rabbit anti-COX5B Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Wed 3 Apr
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

COX5B

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Between 25 and 75

Alternative Names:

COXVB; Cytochrome c oxidase polypeptide Vb; cytochrome c oxidase polypeptide VB, mitochondrial; cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase subunit Vb; epididymis secretory sperm binding protein

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Product Description:

Rabbit anti-COX5B Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse COX5B by we
Detection of human and mouse COX5B by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-COX5B antibody A305-523A (lot A305-523A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human COX5B by western blot
Detection of human COX5B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-COX5B antibody A305-523A (lot A305-523A-1) used for IP at 6 µg per reaction. COX5B was also immunoprecipitated by rabbit anti-COX5B antibody A305-524A. For blotting immunoprecipitated COX5B, A305-523A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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