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Rabbit anti-IDH3B Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Fri 5 Apr
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Between 75 and 125

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Product Description:

Rabbit anti-IDH3B Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse IDH3B by we
Detection of human and mouse IDH3B by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IDH3B antibody A305-501A (lot A305-501A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human IDH3B by western blot
Detection of human IDH3B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IDH3B antibody A305-501A (lot A305-501A-1) used for IP at 6 µg per reaction. IDH3B was also immunoprecipitated by rabbit anti-IDH3B antibody A305-500A. For blotting immunoprecipitated IDH3B, A305-501A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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