Rabbit anti-YKT6 Antibody, Affinity Purified
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Rabbit anti-YKT6 Antibody, Affinity Purified - 100 µg
Antibodies
R-SNARE, SNARE protein Ykt6, synaptobrevin homolog YKT6, YKT6 v-SNARE protein, YKT6, S. cerevisiae, homolog of
Human, Mouse
Between 125 and 175
2 - 8°C
Rabbit
Whole IgG
Polyclonal
12352203
Detection of human and mouse YKT6 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-YKT6 antibody A305-479A (lot A305-479A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human YKT6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-YKT6 antibody A305-479A (lot A305-479A-1) used for IP at 6 µg per reaction. YKT6 was also immunoprecipitated by rabbit anti-YKT6 antibody A305-480A. For blotting immunoprecipitated YKT6, A305-479A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.