CI-23k, CI23KD, CI-23kD, complex I 23kDa subunit, complex I-23kD, NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, NADH-ubiquinone oxidoreductase 23 kD
Between 25 and 75
2 - 8°C
Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFS8 antibody A305-437A (lot A305-437A-1) used for IP at 6 µg per reaction. NDUFS8 was also immunoprecipitated by rabbit anti-NDUFS8 antibody A305-438A. For blotting immunoprecipitated NDUFS8, A305-437A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.