B17, CI, CI-B17, complex I, mitochondrial respiratory chain, B17 subunit, complex I-B17, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6, NADH-ubiquinone oxidoreductase B17 subunit,
Between 78 and 128
2 - 8°C
Detection of human and mouse NDUFB6 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NDUFB6 antibody A305-436A (lot A305-436A-1) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human NDUFB6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFB6 antibody A305-436A (lot A305-436A-1) used for IP at 6 µg per reaction. NDUFB6 was also immunoprecipitated by rabbit anti-NDUFB6 antibody A305-435A. For blotting immunoprecipitated NDUFB6, A305-436A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.