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Rabbit anti-NF1 Antibody, Affinity Purified

Catalogue number:
A304-932A-T
Size:
10 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to NF1 immobilized on solid support. The epitope recognized by A304-932A-T maps to a region between residue 150 to 200 of human Neurofibromin 1 using the numbering given in entry NP_001035957.1 (GeneID 4763). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Product Type:

Antibodies

Alternative Names:

neurofibromatosis 1, neurofibromatosis-related protein NF-1, neurofibromin, NFNS, truncated neurofibromin 1, VRNF, WSS

Species Reactivity:

Human

Immunogen:

Between 150 and 200

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human NF1 by western blot o

Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody A304-932A-T (lot A304-932A-T-1) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibodies A304-931A and A300-140A. For blotting immunoprecipitated NF1, A304-932A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.