Antibody was affinity purified using an epitope specific to ISY1 immobilized on solid support. The epitope recognized by A304-927A-T maps to a region between residue 200 to 250 of human Pre-mRNA-splicing factor ISY1 homolog using the numbering given in entry Q9ULR0.3 (GeneID 57461). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
FSAP33, functional spliceosome-associated protein 33, pre-mRNA-splicing factor ISY1 homolog
Between 200 and 250
2 - 8°C
Detection of human ISY1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ISY1 antibody A304-927A-T (lot A304-927A-T-1) used for IP at 6 µg per reaction. ISY1 was also immunoprecipitated by rabbit anti-ISY1 antibody BL19483. For blotting immunoprecipitated ISY1, A304-927A-T was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.