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Rabbit anti-TJP3/ZO-3 Antibody, Affinity Purified - A304-926A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-TJP3/ZO-3 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A304-926A
Name:
Rabbit anti-TJP3/ZO-3 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to TJP3/ZO-3 immobilized on solid support.The epitope recognized by A304-926A maps to a region between residue 883 to 933 of human Tight junction protein ZO-3 using the numbering given in entry O95049.2 (GeneID 27134).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
Tight junction protein 3, tight junction protein 3 (zona occludens 3), tight junction protein ZO-3, ZO3, ZO-3, zona occludens protein 3, zonula occludens protein 3
Species Reactivity:
Human
Immunogen:
Between 883 and 933
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human TJP3/ZO-3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TJP3/ZO-3 antibody A304-926A (lot A304-926A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human TJP3/ZO-3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TJP3/ZO-3 antibody A304-926A (lot A304-926A-1) used for IP at 6 µg per reaction. TJP3/ZO-3 was also immunoprecipitated by rabbit anti-TJP3/ZO-3 antibody A304-925A. For blotting immunoprecipitated TJP3/ZO-3, A304-926A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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