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Rabbit anti-Septin 10/SEPT10 Antibody, Affinity Purified - A304-915A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Septin 10/SEPT10 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A304-915A
Name:
Rabbit anti-Septin 10/SEPT10 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Septin 10/SEPT10 immobilized on solid support.The epitope recognized by A304-915A maps to a region between residue 404 to 454 of human Septin-10 using the numbering given in entry Q9P0V9.2 (GeneID 151011).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
septin-10
Species Reactivity:
Human
Immunogen:
Between 404 and 454
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Septin 10/SEPT10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Septin 10/SEPT10 antibody A304-915A (lot A304-915A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Septin 10/SEPT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Septin 10/SEPT10 antibody A304-915A (lot A304-915A-1) used for IP at 6 µg per reaction. Septin 10/SEPT10 was also immunoprecipitated by rabbit anti-Septin 10/SEPT10 antibody A304-916A. For blotting immunoprecipitated Septin 10/SEPT10, A304-915A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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