Antibody was affinity purified using an epitope specific to HOOK3 immobilized on solid support. The epitope recognized by A304-906A maps to a region between residue 425 to 475 of human Protein Hook homolog 3 using the numbering given in entry Q86VS8.2 (GeneID 84376). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
hHK3, h-hook3, HK3, hook homolog 3, protein Hook homolog 3
Between 425 and 475
2 - 8°C
Detection of human and mouse HOOK3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HOOK3 antibody A304-906A (lot A304-906A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human HOOK3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HOOK3 antibody A304-906A (lot A304-906A-1) used for IP at 6 µg per reaction. HOOK3 was also immunoprecipitated by rabbit anti-HOOK3 antibody A304-905A. For blotting immunoprecipitated HOOK3, A304-906A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.