Antibody was affinity purified using an epitope specific to CNOT10 immobilized on solid support. The epitope recognized by A304-899A-T maps to a region between residue 600 to 650 of human CCR4-NOT transcription complex subunit 10 using the numbering given in entry Q9H9A5.1 (GeneID 25904).
CCR4-NOT transcription complex subunit 10
Between 600 and 650
2 - 8°C
Detection of human and mouse CNOT10 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CNOT10 antibody A304-899A-T (lot A304-899A-T-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.