Antibody was affinity purified using an epitope specific to PDXDC1 immobilized on solid support. The epitope recognized by A304-890A-T maps to a region between residue 738 to 788 of human Pyridoxal-dependent decarboxylase domain-containing protein 1 using the numbering given in entry Q6P996.2 (GeneID 23042). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
LP8165, pyridoxal-dependent decarboxylase domain-containing protein 1
Between 738 and 788
2 - 8°C
Detection of human PDXDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDXDC1 antibody A304-890A-T (lot A304-890A-T-1) used for IP at 6 µg per reaction. PDXDC1 was also immunoprecipitated by rabbit anti-PDXDC1 antibody A304-889A. For blotting immunoprecipitated PDXDC1, A304-890A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.