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Rabbit anti-RUFY1 Antibody, Affinity Purified

Catalogue number:
A304-884A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to RUFY1 immobilized on solid support. The epitope recognized by A304-884A maps to a region between residue 658 to 708 of human RUN and FYVE domain-containing protein 1 using the numbering given in entry Q96T51.2 (GeneID 80230). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

FYVE-finger protein EIP1, la-binding protein 1, rab4-interacting protein, RABIP4, RUN and FYVE domain-containing protein 1, ZFYVE12, zinc finger FYVE domain-containing protein 12

Species Reactivity:

Human, Mouse

Immunogen:

Between 658 and 708

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse RUFY1 by we

Detection of human and mouse RUFY1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RUFY1 antibody A304-884A (lot A304-884A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RUFY1 by western blot

Detection of human RUFY1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RUFY1 antibody A304-884A (lot A304-884A-1) used for IP at 6 µg per reaction. RUFY1 was also immunoprecipitated by rabbit anti-RUFY1 antibody A304-883A. For blotting immunoprecipitated RUFY1, A304-884A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.