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Rabbit anti-RUFY1 Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Wed 27 Mar
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

RUFY1

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Between 658 and 708

Alternative Names:

rab4-interacting protein; zinc finger FYVE domain-containing protein 12; ZFYVE12; RABIP4; la-binding protein 1; FYVE-finger protein EIP1; RUN and FYVE domain-containing protein 1

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Product Description:

Rabbit anti-RUFY1 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse RUFY1 by we
Detection of human and mouse RUFY1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RUFY1 antibody A304-884A (lot A304-884A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human RUFY1 by western blot
Detection of human RUFY1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RUFY1 antibody A304-884A (lot A304-884A-1) used for IP at 6 µg per reaction. RUFY1 was also immunoprecipitated by rabbit anti-RUFY1 antibody A304-883A. For blotting immunoprecipitated RUFY1, A304-884A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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