Antibody was affinity purified using an epitope specific to RUFY1 immobilized on solid support. The epitope recognized by A304-883A maps to a region between residue 275 to 325 of human RUN and FYVE domain-containing protein 1 using the numbering given in entry Q96T51.2 (GeneID 80230). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
FYVE-finger protein EIP1, la-binding protein 1, rab4-interacting protein, RABIP4, RUN and FYVE domain-containing protein 1, ZFYVE12, zinc finger FYVE domain-containing protein 12
Between 275 and 325
2 - 8°C
Detection of human RUFY1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RUFY1 antibody A304-883A (lot A304-883A-1) used for IP at 6 µg per reaction. RUFY1 was also immunoprecipitated by rabbit anti-RUFY1 antibody A304-884A. For blotting immunoprecipitated RUFY1, A304-883A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.