Antibody was affinity purified using an epitope specific to PHKB immobilized on solid support. The epitope recognized by A304-881A-M maps to a region between residue 650 to 700 of human Phosphorylase b kinase regulatory subunit beta using the numbering given in entry Q93100.3 (GeneID 5257).
phosphorylase b kinase regulatory subunit beta, phosphorylase kinase beta-subunit, phosphorylase kinase subunit beta, phosphorylase kinase, beta
Between 650 and 700
2 - 8°C
Detection of human PHKB by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHKB antibody A304-881A-M (lot A304-881A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.