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Rabbit anti-PHKB Antibody, Affinity Purified

Catalogue number:
A304-881A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to PHKB immobilized on solid support. The epitope recognized by A304-881A maps to a region between residue 650 to 700 of human Phosphorylase b kinase regulatory subunit beta using the numbering given in entry Q93100.3 (GeneID 5257). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

phosphorylase b kinase regulatory subunit beta, phosphorylase kinase beta-subunit, phosphorylase kinase subunit beta, phosphorylase kinase, beta

Species Reactivity:

Human

Immunogen:

Between 650 and 700

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human PHKB by western blot.

Detection of human PHKB by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHKB antibody A304-881A (lot A304-881A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PHKB by western blot

Detection of human PHKB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHKB antibody A304-881A (lot A304-881A-1) used for IP at 6 µg per reaction. PHKB was also immunoprecipitated by rabbit anti-PHKB antibody BL19144. For blotting immunoprecipitated PHKB, A304-881A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.