Antibody was affinity purified using an epitope specific to SCYL2 immobilized on solid support. The epitope recognized by A304-880A-T maps to a region between residue 850 to 900 of human SCY1-like protein 2 using the numbering given in entry Q6P3W7.1 (GeneID 55681).
coated vesicle-associated kinase of 104 kDa, CVAK104, SCY1-like 2, SCY1-like protein 2, SCY1-like, kinase-like 2
Between 850 and 900
2 - 8°C
Detection of human and mouse SCYL2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCYL2 antibody A304-880A-T (lot A304-880A-T-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.