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Rabbit anti-ZFR Antibody, Affinity Purified - A304-868A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-ZFR Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A304-868A
Name:
Rabbit anti-ZFR Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to ZFR immobilized on solid support.The epitope recognized by A304-868A maps to a region between residue 1 to 50 of human Zinc finger RNA-binding protein using the numbering given in entry Q96KR1.2 (GeneID 51663).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
hZFR, M-phase phosphoprotein homolog, SPG71, ZFR1, zinc finger RNA-binding protein
Species Reactivity:
Human
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human ZFR by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZFR antibody A304-868A (lot A304-868A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human ZFR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZFR antibody A304-868A (lot A304-868A-1) used for IP at 6 µg per reaction. ZFR was also immunoprecipitated by rabbit anti-ZFR antibody BL18962. For blotting immunoprecipitated ZFR, A304-868A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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