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Rabbit anti-AJUBA/JUB Antibody, Affinity Purified - A304-867A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-AJUBA/JUB Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A304-867A-T
Name:
Rabbit anti-AJUBA/JUB Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to AJUBA/JUB immobilized on solid support.The epitope recognized by A304-867A-T maps to a region between residue 75 to 125 of human Protein ajuba using the numbering given in entry Q96IF1.1 (GeneID 84962).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
JUB, jub, ajuba homolog, LIM domain-containing protein ajuba
Species Reactivity:
Human
Immunogen:
Between 75 and 125
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human AJUBA/JUB by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AJUBA/JUB antibody A304-867A-T (lot A304-867A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human AJUBA/JUB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AJUBA/JUB antibody A304-867A-T (lot A304-867A-T-1) used for IP at 6 µg per reaction. AJUBA/JUB was also immunoprecipitated by rabbit anti-AJUBA/JUB antibody BL18960. For blotting immunoprecipitated AJUBA/JUB, A304-867A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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