Antibody was affinity purified using an epitope specific to NUDCD3 immobilized on solid support. The epitope recognized by A304-866A-T maps to a region between residue 311 to 361 of human NudC domain-containing protein 3 using the numbering given in entry Q8IVD9.3 (GeneID 23386).
nudC domain-containing protein 3, NudCL, NudC-like protein
Between 311 and 361
2 - 8°C
Detection of human and mouse NUDCD3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUDCD3 antibody A304-866A-T (lot A304-866A-T-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.