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Rabbit anti-HMHA1/HA-1 Antibody, Affinity Purified - A304-862A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-HMHA1/HA-1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A304-862A
Name:
Rabbit anti-HMHA1/HA-1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to HMHA1/HA-1 immobilized on solid support.The epitope recognized by A304-862A maps to a region between residue 100 to 150 of human Minor histocompatibility protein HA-1 [Cleaved into: Minor histocompatibility antigen HA-1 using the numbering given in entry Q92619.2 (GeneID 23526).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
HA-1, histocompatibility (minor) HA-1, HLA-HA1, HMHA1, minor histocompatibility antigen HA-1, minor histocompatibility protein HA-1, rho GTPase-activating protein 45
Species Reactivity:
Human
Immunogen:
Between 100 and 150
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human HMHA1/HA-1 by western blot. Samples: Whole cell lysate (50 µg) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HMHA1/HA-1 antibody A304-862A (lot A304-862A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human HMHA1/HA-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMHA1/HA-1 antibody A304-862A (lot A304-862A-1) used for IP at 6 µg per reaction. HMHA1/HA-1 was also immunoprecipitated by rabbit anti-HMHA1/HA-1 antibody A300-861A. For blotting immunoprecipitated HMHA1/HA-1, A304-862A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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