Antibody was affinity purified using an epitope specific to MIA3/TANGO immobilized on solid support. The epitope recognized by A304-860A maps to a region between residue 350 to 400 of human Melanoma inhibitory activity protein 3 using the numbering given in entry Q5JRA6.1 (GeneID 375056). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ARNT, C219-reactive peptide, D320, melanoma inhibitory activity family, member 3, melanoma inhibitory activity protein 3, MIA family member 3, ER export factor, TANGO, TANGO1, transport and Golgi organization protein 1, transport and Golgi organization pr
Between 350 and 400
2 - 8°C
Detection of human MIA3/TANGO by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIA3/TANGO antibody A304-860A (lot A304-860A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.