Antibody was affinity purified using an epitope specific to RPAP3 immobilized on solid support. The epitope recognized by A304-854A-T maps to a region between residue 400 to 450 of human RNA polymerase II-associated protein 3 using the numbering given in entry Q9H6T3.2 (GeneID 79657).
- Applications: WB, IP
hSpagh, RNA polymerase II-associated protein 3, Tah1
Between 400 and 450
2 - 8°C
Detection of human RPAP3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPAP3 antibody A304-854A-T (lot A304-854A-T-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.