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Rabbit anti-RPAP3 Antibody, Affinity Purified - A304-854A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RPAP3 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A304-854A
Name:
Rabbit anti-RPAP3 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to RPAP3 immobilized on solid support. The epitope recognized by A304-854A maps to a region between residue 400 to 450 of human RNA polymerase II-associated protein 3 using the numbering given in entry Q9H6T3.2 (GeneID 79657). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
hSpagh, RNA polymerase II-associated protein 3, Tah1
Species Reactivity:
Human
Immunogen:
Between 400 and 450
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human RPAP3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPAP3 antibody A304-854A (lot A304-854A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human RPAP3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPAP3 antibody A304-854A (lot A304-854A-1) used for IP at 6 µg per reaction. RPAP3 was also immunoprecipitated by rabbit anti-RPAP3 antibody BL18921. For blotting immunoprecipitated RPAP3, A304-854A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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