Antibody was affinity purified using an epitope specific to DPP9/DPRP2 immobilized on solid support. The epitope recognized by A304-851A maps to a region between residue 775 to 825 of human Dipeptidyl peptidase 9 using the numbering given in entry Q86TI2.3 (GeneID 91039). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
dipeptidyl peptidase 9, Dipeptidyl peptidase IV-related protein 2, dipeptidyl peptidase IV-related protein-2, dipeptidyl peptidase IX, dipeptidyl peptidase-like protein 9, DP9, DPLP9, DPP IX, DPRP2, DPRP-2
Between 775 and 825
2 - 8°C
Detection of human DPP9/DPRP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DPP9/DPRP2 antibody A304-851A (lot A304-851A-1) used for IP at 6 µg per reaction. DPP9/DPRP2 was also immunoprecipitated by rabbit anti-DPP9/DPRP2 antibody BL18907. For blotting immunoprecipitated DPP9/DPRP2, A304-851A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.