Antibody was affinity purified using an epitope specific to UBAP1/NAG20 immobilized on solid support. The epitope recognized by A304-848A maps to a region between residue 25 to 75 of human Ubiquitin-associated protein 1 using the numbering given in entry Q9NZ09.1 (GeneID 51271). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
NAG20, nasopharyngeal carcinoma-associated gene 20 protein, UAP, UBAP, UBAP-1, ubiquitin-associated protein 1
Between 25 and 75
2 - 8°C
Detection of human UBAP1/NAG20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBAP1/NAG20 antibody A304-848A (lot A304-848A-1) used for IP at 6 µg per reaction. UBAP1/NAG20 was also immunoprecipitated by rabbit anti-UBAP1/NAG20 antibody BL18890. For blotting immunoprecipitated UBAP1/NAG20, A304-848A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.