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Bromodomain-Containing (BRD) Antibodies
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Rabbit anti-POLR1A/RPA194 Antibody, Affinity Purified - A304-845A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-POLR1A/RPA194 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A304-845A
Name:
Rabbit anti-POLR1A/RPA194 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to POLR1A/RPA194 immobilized on solid support.The epitope recognized by A304-845A maps to a region between residue 1350 to 1400 of human DNA-directed RNA polymerase I subunit RPA1 using the numbering given in entry O95602.2 (GeneID 25885).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

IP

Product Type:
Antibodies
Alternative Names:
A190, AFDCIN, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, DNA-directed RNA polymerase I subunit A1, DNA-directed RNA polymerase I subunit RPA1, polymerase (RNA) I polypeptide A, 194kDa, polymerase (RNA) I subuni
Species Reactivity:
Human
Immunogen:
Between 1350 and 1400
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human POLR1A/RPA194 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-POLR1A/RPA194 antibody A304-845A (lot A304-845A-1) used for IP at 6 µg per reaction. POLR1A/RPA194 was also immunoprecipitated by rabbit anti-POLR1A/RPA194 antibody A304-846A. For blotting immunoprecipitated POLR1A/RPA194, A304-846A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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