Antibody was affinity purified using an epitope specific to MMACHC immobilized on solid support. The epitope recognized by A304-541A-T maps to a region between residue 175 to 225 of human Methylmalonic Aciduria and Homocystinuria Type C Protein using the numbering given in entry NP_056321.2 (GeneID 25974). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cblC, cyanocobalamin reductase (cyanide-eliminating), methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria, methylmalonic aciduria and homocystinuria type C protein
Between 175 and 225
2 - 8°C
Detection of human MMACHC by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MMACHC antibody A304-541A-T (lot A304-541A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.