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Rabbit anti-Endoglycan Antibody, Affinity Purified

Catalogue number:
A304-484A-T
Size:
10 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Endoglycan immobilized on solid support. The epitope recognized by A304-484A-T maps to a region between residue 300 to 350 of human Endoglycan using the numbering given in entry NP_056535.1 (GeneID 50512). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

EG, endoglycan, PODLX2, podocalyxin-like protein 2

Species Reactivity:

Human

Immunogen:

Between 300 and 350

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Endoglycan by western

Detection of human Endoglycan by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Endoglycan antibody A304-484A-T (lot A304-484A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Endoglycan by western

Detection of human Endoglycan by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Endoglycan antibody A304-484A-T (lot A304-484A-T-1) used for IP at 6 µg per reaction. Endoglycan was also immunoprecipitated by rabbit anti-Endoglycan antibody BL16933. For blotting immunoprecipitated Endoglycan, A304-484A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.