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Rabbit anti-Endoglycan Antibody, Affinity Purified - A304-484A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Endoglycan Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A304-484A-T
Name:
Rabbit anti-Endoglycan Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Endoglycan immobilized on solid support.The epitope recognized by A304-484A-T maps to a region between residue 300 to 350 of human Endoglycan using the numbering given in entry NP_056535.1 (GeneID 50512).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
EG, endoglycan, PODLX2, podocalyxin-like protein 2
Species Reactivity:
Human
Immunogen:
Between 300 and 350
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Endoglycan by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Endoglycan antibody A304-484A-T (lot A304-484A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human Endoglycan by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Endoglycan antibody A304-484A-T (lot A304-484A-T-1) used for IP at 6 µg per reaction. Endoglycan was also immunoprecipitated by rabbit anti-Endoglycan antibody BL16933. For blotting immunoprecipitated Endoglycan, A304-484A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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