Antibody was affinity purified using an epitope specific to Protocadherin-7 immobilized on solid support. The epitope recognized by A304-472A-T maps to a region between residue 1019 to 1069 of human Protocadherin-7 using the numbering given in entry NP_002580.2 (GeneID 5099). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
BHPCDH, BH-Pcdh, BH-protocadherin (brain-heart), brain-heart protocadherin, PPP1R120, protein phosphatase 1, regulatory subunit 120, protocadherin-7
Between 1019 and 1069
2 - 8°C
Detection of human and mouse Protocadherin-7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Protocadherin-7 antibody A304-472A-T (lot A304-472A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.