Antibody was affinity purified using an epitope specific to PCDH19 immobilized on solid support. The epitope recognized by A304-468A-T maps to a region between residue 1098 to 1148 of human Protocadherin-19 using the numbering given in entry NP_001171809.1 (GeneID 57526). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
EFMR, EIEE9, protocadherin-19
Between 1098 and 1148
2 - 8°C
Detection of human and mouse PCDH19 by western blot. Samples: Whole cell lysate (10µg or 50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PCDH19 antibody A304-468A-T (lot A304-468A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.