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Rabbit anti-Nicastrin Antibody, Affinity Purified - A304-464A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Nicastrin Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A304-464A-T
Name:
Rabbit anti-Nicastrin Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Nicastrin immobilized on solid support.The epitope recognized by A304-464A-T maps to a region between residue 659 to 709 of human Nicastrin using the numbering given in entry NP_056146.1 (GeneID 23385).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
anterior pharynx-defective 2, ATAG1874, nicastrin
Species Reactivity:
Human
Immunogen:
Between 659 and 709
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Nicastrin by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody A304-464A-T (lot A304-464A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Nicastrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody A304-464A-T (lot A304-464A-T-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated Nicastrin, A304-464A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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