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Rabbit anti-Nicastrin Antibody, Affinity Purified

Catalogue number:
A304-464A-T
Size:
10 µg
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Antibody was affinity purified using an epitope specific to Nicastrin immobilized on solid support. The epitope recognized by A304-464A-T maps to a region between residue 659 to 709 of human Nicastrin using the numbering given in entry NP_056146.1 (GeneID 23385). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

anterior pharynx-defective 2, ATAG1874, nicastrin

Species Reactivity:

Human

Immunogen:

Between 659 and 709

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Nicastrin by western

Detection of human Nicastrin by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody A304-464A-T (lot A304-464A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Nicastrin by western

Detection of human Nicastrin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody A304-464A-T (lot A304-464A-T-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated Nicastrin, A304-464A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.