Antibody was affinity purified using an epitope specific to RNA Polymerase II, Phospho (S5) immobilized on solid support. Immunogen for A304-408A-M was a phosphorylated synthetic peptide, which represented a portion of human RNA Polymerase II (GeneID 5430) around serine 5 of the C-terminal repeat YSPTSPS. of human RNA Polymerase II using the numbering given in entry NP_000928.1 (GeneID 5430). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, POLRA, polyme
surrounding serine 5
2 - 8°C
Detection of human Phospho RNA Polymerase II (S5) by western blot. Samples: Whole cell lysate 50 µ g) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S5) antibody A304-408A -M (lot A304-408A- M- 2) was used for WB at 1:1000 (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody A300-653A was used (lower panel). Detection: Chemiluminescence with exposure time of 10 seconds.