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Rabbit anti-DHPS Antibody, Affinity Purified - A304-375A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DHPS Antibody, Affinity Purified
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Size: 10 µg
£89.00
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Catalogue number:
A304-375A-T
Name:
Rabbit anti-DHPS Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to DHPS immobilized on solid support.The epitope recognized by A304-375A-T maps to a region between residue 319 to 369 of human Deoxyhypusine Synthase using the numbering given in entry NP_001921.1 (GeneID 1725).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
deoxyhypusine synthase, DHS, DS, MIG13, migration-inducing gene 13
Species Reactivity:
Human, Mouse
Immunogen:
Between 319 and 369
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse DHPS by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-DHPS antibody A304-375A-T (lot A304-375A-T-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human DHPS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-DHPS antibody A304-375A-T (lot A304-375A-T-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated DHPS, A304-375A-T was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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